Molecular neurodevelopment: An in vivo31P-1H MRSI study To view the full article click here to login Gerald Goldstein1, Kanagasabai Panchalingam2, Richard J. McClure2, Jeffrey A. Stanley6,Vince D. Calhoun7,8, Godfrey D. Pearlson9, and Jay W. Pettegrew2,3,4,5 1 VA Pittsburgh Healthcare System, Pittsburgh, PA2 Department of Psychiatry, University of Pittsburgh School of Medicine, University of Pittsburgh,Pittsburgh, PA3…
Read More23Na and 7Li NMR Studies of Mammalian Cells: Assessment of Cation Transport and Cytoskeletal Structure with Application to Manic Depressive Disease
23Na and 7Li NMR Studies of Mammalian Cells: Assessment of Cation Transport and Cytoskeletal Structure with Application to Manic Depressive Disease To view the full article click here to login J.W Pettegrew and D.E. Woessner I. IntroductionA. Sodium BiologyThe regulation of Na+ trans-membrane fluxes and intracellular Na+concentrations, is of fundamental importance to human biological processes.Exchange…
Read MoreSolid State 31p and 27 Al NMR Studies of Model Membranes and Mammalian Brain: Possible Implications for Alzheimer’s Disease
J. W. Pettegrew and K. Panchalingam Introduction and Background A. Chemical Characteristics Aluminum is the third most abundant element in the earth’s crust (over 8% (W/W) of the earth’s crust) following oxygen (46.6%) and silicon (27.7%). Aluminum bonds to oxygen and silicon in aluminosilicate granites and clays (Bailar et al 1984; Cotton et al…
Read More31p NMR of Brain Aging and Alzheimer’s Disease
31p NMR of Brain Aging and Alzheimer’s Disease
Read MoreA Preliminary 31p MRS Study of Autism: Evidence for Undersynthesis and Increased Degradation of Brain Membranes – LOGIN TO VIEW
To view the full article click here to login Nancy J. Minshew, Gerald Goldstein, Stephen M. Dombrowski,Kanagasabai Panchalingam, and Jay W. Pettegrew In this pilot study, brain high energy phosphate and membrane phospholipid metabolism wereinvestigated in the dorsal prefrontal cortex of II high-functioning autistic adolescent andyoung adult men (the age range is 12-36 years) and…
Read MoreAβ peptide interactions with isoflurane, propofol, thiopental and combined thiopental with halothane: A NMR study
Aβ peptide is the major component of senile plaques (SP) which accumulates in AD (Alzheimer’s disease) brain. Reports from different laboratories indicate that anesthetics interact with Aβ peptide and induce Aβ oligomerization. The molecular mechanism of Aβ peptide interactions with these anesthetics was not determined. We report molecular details for the interactions of uniformly 15N labeled Aβ40 with different anesthetics using 2D nuclear magnetic resonance (NMR) experiments. At high concentrations both isoflurane and propofol perturb critical amino acid residues (G29, A30 and I31) of Aβ peptide located in the hinge region leading to Aβ oligomerization. In contrast, these three specific residues do not interact with thiopental and subsequently no Aβ oligomerization was observed. However, studies with combined anesthetics (thiopental and halothane), showed perturbation of these residues (G29, A30 and I31) and subsequently Aβ oligomerization was found. Perturbation of these specific Aβ residues (G29, A30 and I31) by different anesthetics could play an important role to induce Aβ oligomerization.
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