Molecular neurodevelopment: An in vivo31P-1H MRSI study To view the full article click here to login Gerald Goldstein1, Kanagasabai Panchalingam2, Richard J. McClure2, Jeffrey A. Stanley6,Vince D. Calhoun7,8, Godfrey D. Pearlson9, and Jay W. Pettegrew2,3,4,5 1 VA Pittsburgh Healthcare System, Pittsburgh, PA2 Department of Psychiatry, University of Pittsburgh School of Medicine, University of Pittsburgh,Pittsburgh, PA3…
Read More23Na and 7Li NMR Studies of Mammalian Cells: Assessment of Cation Transport and Cytoskeletal Structure with Application to Manic Depressive Disease
23Na and 7Li NMR Studies of Mammalian Cells: Assessment of Cation Transport and Cytoskeletal Structure with Application to Manic Depressive Disease To view the full article click here to login J.W Pettegrew and D.E. Woessner I. IntroductionA. Sodium BiologyThe regulation of Na+ trans-membrane fluxes and intracellular Na+concentrations, is of fundamental importance to human biological processes.Exchange…
Read MoreSolid State 31p and 27 Al NMR Studies of Model Membranes and Mammalian Brain: Possible Implications for Alzheimer’s Disease
J. W. Pettegrew and K. Panchalingam Introduction and Background A. Chemical Characteristics Aluminum is the third most abundant element in the earth’s crust (over 8% (W/W) of the earth’s crust) following oxygen (46.6%) and silicon (27.7%). Aluminum bonds to oxygen and silicon in aluminosilicate granites and clays (Bailar et al 1984; Cotton et al…
Read More31p NMR of Brain Aging and Alzheimer’s Disease
31p NMR of Brain Aging and Alzheimer’s Disease
Read MoreA Preliminary 31p MRS Study of Autism: Evidence for Undersynthesis and Increased Degradation of Brain Membranes – LOGIN TO VIEW
To view the full article click here to login Nancy J. Minshew, Gerald Goldstein, Stephen M. Dombrowski,Kanagasabai Panchalingam, and Jay W. Pettegrew In this pilot study, brain high energy phosphate and membrane phospholipid metabolism wereinvestigated in the dorsal prefrontal cortex of II high-functioning autistic adolescent andyoung adult men (the age range is 12-36 years) and…
Read MoreAβ peptide interactions with isoflurane, propofol, thiopental and combined thiopental with halothane: A NMR study
Aβ peptide is the major component of senile plaques (SP) which accumulates in AD (Alzheimer’s disease) brain. Reports from different laboratories indicate that anesthetics interact with Aβ peptide and induce Aβ oligomerization. The molecular mechanism of Aβ peptide interactions with these anesthetics was not determined. We report molecular details for the interactions of uniformly 15N labeled Aβ40 with different anesthetics using 2D nuclear magnetic resonance (NMR) experiments. At high concentrations both isoflurane and propofol perturb critical amino acid residues (G29, A30 and I31) of Aβ peptide located in the hinge region leading to Aβ oligomerization. In contrast, these three specific residues do not interact with thiopental and subsequently no Aβ oligomerization was observed. However, studies with combined anesthetics (thiopental and halothane), showed perturbation of these residues (G29, A30 and I31) and subsequently Aβ oligomerization was found. Perturbation of these specific Aβ residues (G29, A30 and I31) by different anesthetics could play an important role to induce Aβ oligomerization.
Read MoreMolecular neurodevelopment: An in vivo31P-1H MRSI study
To view the full article click here to login Published in final edited form as: J Int Neuropsychol Soc. 2009 September ; 15(5): 671–683. doi:10.1017/S1355617709990233. Molecular neurodevelopment: An in vivo31P-1H MRSI study Gerald Goldstein1, Kanagasabai Panchalingam2, Richard J. McClure2, Jeffrey A. Stanley6, Vince D. Calhoun7,8, Godfrey D. Pearlson9, and Jay W. Pettegrew2,3,4,5 1VA Pittsburgh Healthcare…
Read MoreDevelopmental Aspects of Working and Associative Memory
Developmental differences between working and long-term associative memory were evaluated through a cross-sectional age difference study based on data from a memory battery’s standardization sample. The scores of 856 children and adolescents ranging from 5 to 17 years of age were compared on memory subtests that assess verbal working and long-term memory. Data were examined using curve fitting and ANOVA procedures that evaluated age group and years of age differences. The major finding was that the developmental trajectories across age differed substantially between the two memory domains. The working memory trajectory was linear until age 11, whereas the long-term memory trajectory was curvilinear with an inflection point at age 8. Both trajectories plateaued after age 11. ANOVAs produced significant interactions between tests of working and associative memory with age, supporting the view that the age trajectories had differing courses. The results are discussed in terms of neurobiological implications for the two memory systems studied.
Read MoreDevelopmental Aspects and Neurobiological Correlates of Working and Associative Memory
Objective: It has been shown that verbal working and associative memory have different developmental trajectories with working memory, taking a linear course from early childhood to adolescence, whereas associative memory takes a curvilinear course asymptoting at about age 12. This study made a determination of whether these trajectories tracked with 2 magnetic resonance spectroscopy imaging (MRSI) variables: phosphocreatine level (PCr) and gray matter percentage (GM%).
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